Bioemulsifier and biosurfactant pdf

Production of biosurfactant and bioemulsifier by Mucor hiemalis. Medium with 5% inoculum and cultivation at 150 rpm, produced 7.73 g/L of a compound with significant surfactant properties, since it showed surface tension of 32 mN/m and an emulsification index of 96%. In this condition, the biomass yield was 2.18 g/L. filexlib. Menezes BF, de Oliveira CFA, Okeke BC, Frankenberger WT Jr (2005) Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol Res 160:249-255. Article Google Scholar Miller RM (1995) Biosurfactant-facilitated remediation of metal contaminated soils. Biosurfactant structural uniqueness resides in the coexistence of a hydrophilic (a sugar or peptide) and a hydrophobic (fatty acid chain) domain in the same molecule, which allows them to occupy the interface of mixed phase systems (e.g., oil/water, air/water, oil/solid/water) and consequently to alter the forces governing the equilibrium
Biosurfactants and emulsifiers are generally found on the surface of the organism producing these compounds as they are secreted extracellularly; they also serve as a protective layer for the microorganisms in adverse environments. Fig. 13.1 Different structures of amphipathic molecules, with differing hydrophilic moieties [ 6 ].
The biosurfactant produced by sample M was extracted and dissolved in 1 ml of demineralized water. The total car-bohydrate and protein concentrations were observed to be 310 and 150 lg/ml, respectively. Sample M showed maximum biosurfactant production at pH 7.0 and 37 C (Fig. 3). The addition of biosurfactant at the concentration
Download chapter PDF 1 Introduction The hydrophilic part of biosurfactant can be an amino acid, a carbohydrate, or a phosphate group, whereas the hydrophobic part is a long chain fatty acid. They can be anionic or neutral. The bioemulsifier was tested for stability at different temperature, pH and NaCl concentrations and was found to be
This strain had a high emulsification activity and biosurfactant production of 78% and 1.8 g/L respectively. The cell free broth containing biosurfactant could reduce the surface tension to 28 mN/m. Fourier transform infrared (FT-IR) spectrum of extracted biosurfactant indicates the presence of carboxyl, hydroxyl and methoxyl functional groups.
Biosurfactants demonstrate low toxicity to freshwater, marine and terrestrial ecosystems and are potential candidates for a variety of environmental applications. Research has largely been focused
Bioemulsifiers can be applied as an additive to stimulate the bioremediation process, however with advanced genetic technologies it is expected that the increase in bioemulsifier concentration during bioremediation would be achieved by the addition of bacteria that overproduce bioemulsifiers. Tensoactive properties of biosurfactants/bioemulsifiers Eight of the 17 isolates that tested positive for biosurfactant production in the drop-collapse test reduced the culture medium surface tension below 40 mN m −1 ( Table 2 ). The eight isolates reduced the surface tension below that of the positive control, 1% SDS, a synthetic surfactant.
Rhamnolipid presence an anionic biosurfactant can form a complex with the cationic compounds (CTAB) that were recognized by the development of dark blue areas across colonies on the agar plates as presented in figure 1 and 2. Essential manufacture of biosurfactant was determined by observing foam in enclosed nutrient broth after 72hrs of
DOI: 10.1016/J.PETROL.2017.10.088 Corpus