Protein purification handbook

This handbook is intended for the general reader interested in the amplification and purification of recombinant proteins and for everyday use at the laboratory bench. The growth in the use of recombinant proteins has increased greatly in recent years, as has the wealth of techniques and products used for their amplification and purification. A handbook for purification of both native and recombinant proteins using affinity chromatography, protein tagging/ labeling and immunoprecipitations. The handbook also features tools for the removal of contaminating reagents, including detergents. The Protein Purification Handbook features the following sections: This booklet provides advice and examples for a smooth path to protein purification. Protein purification varies from simple one-step precipitation procedures to large scale validated production processes. Often more than one purification step is necessary to reach the desired purity. Protein Purification Handbook Cytiva 28-9833-31, pack of 1 ea; find -GE28983331 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Handbook 18-1157-58 2-D Electrophoresis using immobilized pH gradients Principles and Methods 80-6429-60 Antibody Purification Handbook 18-1037-46 The Recombinant Protein Handbook Protein Amplification and Simple Purification 18-1142-75 Protein Purification Handbook 18-1132-29 Ion Exchange Chromatography Principles and Methods 18-1114-21 Protein Purification Handbook 18-1132-29 Gel Filtration Principles and Methods 18-1022-18 Affinity Chromatography Principles and Methods 18-1022-29 Antibody Purification Handbook 18-1037-46 Cell Separation Media Methodology and Applications 18-1115-69 Ion Exchange Chromatography and Chromatofocusing This purification method is enabled by the specific binding properties of a protein to an immobilized ligand. Since the protein of interest is tightly bound, contaminants can be removed through wash steps, and the bound protein can be stripped (eluted) from the support in a highly purified form. Affinity purification is Protein Purification Handbook 18-1132-29 Gel Filtration Principles and Methods 18-1022-18 Affinity Chromatography Principles and Methods 18-1022-29 Antibody Purification Handbook 18-1037-46 Percoll Methodology and Applications 18-1115-69 Ion Exchange Chromatography & Chromatofocusing Principles and Methods The rapid purification protocols provided in this handbook for affinity chromatography permit the recovery of high levels of pure antibodies in minutes. Large numbers of samples can be processed at the same time. There is negligible hold-up volume; ensuring high solute recovery with minimal non- specific absorptive losses. The Ion Exchange Chromatography Handbook has been recently updated to include the expanding range of Capto™ chromatography resins for lab- and large-scale protein purification. The update brings Sepharose™ and Capto IEX resins together in the same book and the content will ensure you get the most from your purification runs. The preparative purification of histidine-tagged recombinant proteins by Immobilized Metal Affinity Chromatography (IMAC) is both popular and highly effective. IMAC exploits the ability of the amino acid histidine to bind chelated transition metal ions, such as nickel (Ni2+). Histidine-tag purification using IMAC can be performed under both This handbook provides advice and examples for a smooth path to prote