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Running the QIIME tutorial. After you have gotten QIIME working as described, you are ready to try out QIIME. A good first step is to follow the QIIME The tutorials make extensive use of the QIIME 2 command-line interface so reviewing the q2cli docs is recommended. Overview of QIIME 2 Plugin Workflows · Let's Approach: Practicum on 16S analysis with QIIME 2 Alternating lecture and tutorial on command-line software Suggest you pair up with a partner Two eyes are Will discuss only QIIME in this tutorial This tutorial uses QIIME 2 only For today's tutorial, we will use public data from the QIIME2 website. The following is taken directly from the Moving Pictures tutorial and adapted for this data set. QIIME 2's diversity analyses are available through the q2- a. OTU category significance b. Compare categories c. Compare alpha diversity. 10. Heatmaps. G. QIIME Fungal ITS Workflow. 1. Obtain tutorial files.This tutorial explains how to use the QIIME (Quantitative Insights Into Microbial Ecology) Pipeline to process data from high-throughput 16S rRNA sequencing The QIIME tutorials illustrate how to use various features of QIIME. These tutorials take the user through a full analysis of sequencing data.