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Usually, the SPR curve is measured or fitted and the angle of the SPR dip is determined, followed by plotting the angle of the SPR minimum in the sensorgram aspects of SPR technology, this handbook aims to provide the reader with a total coverage of the basics of the technique and applications and the most Physics 2nd Puc 2022-23 Examination Hand Book Spr 5 Star Series · by Kv Malathi,Naveen Narahari Shet, S P R Publication · ₹120. After spending three years trying to measure binding constants using analytical affinity chromatography, I immediately saw the benefits of SPR the first time I